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In silico feedback for in vivo regulation of a gene expression circuit

Author(s):

A. Milias, S. Summers, J. Stewart-Ornstein, I. Zuleta, D. Pincus, H. El-Samad, M. Khammash, J. Lygeros
Conference/Journal:

Nature Biotechnology, vol. 29, no. 12, pp. 11141116, Andreas Milias-Argeitis, Sean Summers, and Jacob Stewart-Ornstein contributed equally to this work.
Abstract:

We show that difficulties in regulating cellular behavior with synthetic biological circuits may be circumvented using in silico feedback control. By tracking a circuit's output in Saccharomyces cerevisiae in real time, we precisely control its behavior using an in silico feedback algorithm to compute regulatory inputs implemented through a genetically encoded light-responsive module. Moving control functions outside the cell should enable more sophisticated manipulation of cellular processes whenever real-time measurements of cellular variables are possible.

Year:

2011
Type of Publication:

(01)Article
Supervisor:

J. Lygeros

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% Autogenerated BibTeX entry
@Article { MilEtal:2011:IFA_3903,
    author={A. Milias and S. Summers and J. Stewart-Ornstein and I. Zuleta and D.
	  Pincus and H. El-Samad and M. Khammash and J. Lygeros},
    title={{In silico feedback for in vivo regulation of a gene
	  expression circuit}},
    journal={Nature Biotechnology},
    year={2011},
    volume={29},
    number={12},
    pages={11141116},
    month=dec,
    url={http://control.ee.ethz.ch/index.cgi?page=publications;action=details;id=3903}
}
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