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Microfluidic Platforms for Quantitative Systems Biology

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Abstract:
I will describe an integrated microfluidic platform we have recently developed that allows us to gather systems-scale quantitative data. Our MITOMI platform measures hundreds to thousands of protein interactions in parallel on a single microfluidic device. We have shown that MITOMI can be used to measure a variety of interactions, including protein-protein, protein-DNA, and protein-RNA. MITOMI is also the only platform that integrates on-chip protein synthesis, purification, and characterization. We have recently extended the MITOMI concept to kinetic rate measurements or k-MITOMI. With our k-MITOMI platform we are able to measure 768 association and dissociation events in parallel on a single platform. I will discuss these recent developments in high-throughput quantitative protein biochemistry and give specific examples of how we applied our MITOMI platforms to the characterization of transcription factors and their interactions with DNA.

Type of Seminar:
IfA BISON Seminar
Speaker:
Prof Sebastian Maerkl
Laboratory of Biological Network Characterization, EPFL Lausanne, Switzerland
Date/Time:
Sep 27, 2011   13:30
Location:

ETL K25
Contact Person:

Elias August
No downloadable files available.
Biographical Sketch: